Proteins and mRNAs identified and affected by DA agonists in the hypothalamus of goldfish.

Proteins were determined by iTRAQ as being significantly (FDR-adj p<0.05) differentially regulated in the hypothalamus of female goldfish treated with either SKF 38393 (SKF) or LY 171555 (LY) agonists. This table also show the cDNAs corresponding to the proteins identified by microarray analysis as significantly (q<5%) affected by the same treatments. Negative values indicate a decrease relative to control. Absent values indicate either that no significant change was detected, or, in the case of the mRNAs, that the corresponding cDNA was not present on the array. mRNAs were annotated using Blast2GO's Blast Descriptor Annotator with default values except the Blast ExpectValue was changed from 1.0E-3 to 1.0E-5. Following the Mapping step, the Annotation Configuration E-Value-Hit-Filter was changed from 1.0E-6 (default) to 1.0E-8 to increase the likelihood of proper GO annotation. Duplicates were assessed on the basis of sequence comparison and removed if a similar expression was observed. In the case where different expression profiles were seen (*), both ESTs were included, as it is possible that the sequences correspond to separate genes.