Development of new high quality mock communities using a method that measures the amount of genomic DNA.

Multi-process metagenomic analysis is prone to experimental bias, and improving its reliability is an urgent issue. Therefore, it has been proposed to use control samples, called mock communities, which can measure the accuracy of the experimental process, for metagenomic sequencing analysis. Our goal is to develop new mock communities suitable for various microbiome studies, and we focused on a method to measure the amount of genomic DNA in bacterial cells to produce high-quality mock communities. Shotgun sequencing was used to validate the relative abundance of multiple mixed strains. Our results showed that this method is suitable for achieving our goal.