Effect of cadmium on enzyme activities of the acetoclastic pathway upper part from <i>Methanosarcina acetivorans</i>.

All activities were determined by using freshly prepared cytosolic fraction as described in the Methods section. Values are the mean ± SD of at least three independent preparations. Control activities were for AK: 0.75±0.21 U/mg protein (n = 4); for Pta: 1.48±0.8 U/mg protein (n = 4); for CODH/AcCoA synthase with acetyl-CoA: 0.37 U±0.12 U/mg protein (n = 5); and with CO: 0.68±0.11 U/mg protein (n = 3); CA: 26±12 U/mg protein. *P<0.05 vs control for independent samples; **P<0.05 vs control for paired samples. ND: Not determined.