miRNA expression profiling and identification of a miRNA regulator for muscle regeneration in the ischemic limb with proanthocyanidins treatment of adult mice

Using the highly sensitive miRNA array, we screened 411 differentially expressed genes microRNAs in the ischemic muscle with proanthocyanidins treatment and we explored the functions of these miRNAs in the ischemic muslce by Gene Ontology and Kyoto Encyclopedia of Genes annotation. The enrichment results indicated that these miRNAs mainly participated in the muscle regeneration process. Furthermore, the quantitative real-time polymerase chain reaction, immunohistochemistry and dual luciferase reporter assay results showed that miR-133b-3p is a specific regulator of MKP1/ p38-MAPK signal axis, offering a novel therapeutic opportunity for limb ischemic injury. In this study, six samples from steady-state mice were used to acquire the miRNA expression profiling and the function of the abundant miRNAs in the ischemic muscle with proanthocyanidins treatment were analyzed by bioinformatic methods,finally, miR-133b-3p was experimentally validated to be a regulator of MPK1.