Cape Darnley Early-Autumn Phytoplankton Bloom, March 2012

These data relate to a large-scale early-autumn phytoplankton bloom that occurred off Cape Darnley, East Antarctica, in March 2012. The bloom was detected by Dr Jan Lieser (Antarctic Climate and Ecosystems Cooperative Research Centre, ACE-CRC) through MODIS satellite and was opportunistically sampled from RSV Aurora Australis using the uncontaminated seawater line. Samples were analysed for protist species and abundances using light and scanning electron microscopy, and pigment analyses were conducted using high performance liquid chromatography. Additional water samples were taken for dissolved nutrient analyses. Specific details of the files are: Cape Darnley Protist Counts Samples were preserved with 1 % vol:vol Lugols iodine and stored in glass bottles in the dark at 4 degrees C. Protists were identified and counted using phase and Nomarski interference optics using Olympus IX71 and IX81 inverted microscopes at 400X to 640X magnification. Bright field optics were also used to discriminate taxa that contained chloroplasts. Protistan taxa were counted in 20 randomly chosen fields of view, except for highly abundant taxa that were counted in a subset of the field of view defined by an ocular quadrant (Whipple grid). Cell biovolumes and carbon conversion statistics were used to calculate the cell biomass of protistan taxa/groups. Cape Darnley Fluorometer Calibration Fluorometer measurements from the ships underway system were calibrated using chlorophyll a readings determined through high performance liquid chromatography. A linear relationship was established between fluorometer v HPLC chlorophyll a measurements at the same sites. The linear equation was then used to convert all underway fluorometry data from the voyage. Cape Darnley Bloom HPLC Pigments CHEMTAX summary Major phytoplankton groups at each site determined through analysis of pigments using high performance liquid chromatography and CHEMTAX. Methods were according to that of Wright et al. (2010). Cape Darnley Bloom Nutrients Dissolved nutrient concentrations. Samples were analysed by the Department of Primary Industries, Parks, Water and Environment, 18 St. Johns Avenue, Newtown, Tasmania 7008. Cape Darnley Underway Data VOYAGE_04_0_201112 Raw underway data from Aurora Australis in the bloom region Cape Darnley Underway Data Maps Maps of the underway data in the bloom region Quality: We were only able to obtain samples through the uncontaminated seawater line, with the intake positioned at 4.8 m below the ship. However, we think the majority of the bloom was at the surface associated with ice. Our data therefore likely under-represents the true surface phenomenon. 2018-10-10 The original files was reformatted to fit OBIS/GBFI/IPT Biodiversity.AQ tracking standards, and a new datasheet \"CapeDarnelyBloom_2012.csv\" was created. The new dataset contains datasetID, occurrenceID, recordNumber, verbatimLatitude, verbatimLongitude, decimalLatitude, decimalLongitude, locationID, locality, eventRemark, eventDate, year, month, basisOfRecord, occurrenceStatus, organismQuantity, organismQuantityType, preparations, institutionCode, continent. The lowest taxonomical rank of the species identified that could be determined is provided, after matched in WoRMS (World Register of Marine Species).本数据集关联2012年3月南极东部达恩利角（Cape Darnley）海域发生的一次大规模初秋浮游植物水华（phytoplankton bloom）事件。此次水华由简·利瑟博士（南极气候与生态系统合作研究中心，ACE-CRC）通过MODIS卫星探测发现，并依托科考补给船“极光南极号”（RSV Aurora Australis）的无污染海水采样管完成机会性采样。研究人员利用光学显微镜与扫描电子显微镜分析了样本中的原生生物（protist）物种组成与丰度，并采用高效液相色谱（high performance liquid chromatography，HPLC）开展色素分析；此外还额外采集了海水样本用于溶解态营养盐分析。 数据集文件具体信息如下： ### 达恩利角原生生物计数数据 样本采用1%体积比的鲁哥氏碘液固定，置于棕色玻璃瓶中于4℃暗处保存。利用奥林巴斯IX71与IX81倒置显微镜，以400×至640×放大倍数，采用相衬及诺马斯基干涉光学系统对原生生物进行鉴定与计数；同时辅以明场光学系统区分含有叶绿体的分类单元。原生生物分类单元的计数在20个随机选取的视野中完成，对于丰度极高的类群，则采用目镜方格（惠普勒网格，Whipple grid）限定的部分视野进行计数。通过细胞生物体积与碳转换系数计算得到原生生物类群的细胞生物量。 ### 达恩利角荧光计校准数据 本船走航系统的荧光计测量数据通过高效液相色谱测得的叶绿素a浓度进行校准。在相同站位处，荧光计测量值与高效液相色谱测得的叶绿素a浓度之间建立了线性相关关系，随后利用该线性方程转换本次科考的全部走航荧光测量数据。 ### 达恩利角水华高效液相色谱色素及CHEMTAX分析汇总数据 通过高效液相色谱色素分析及CHEMTAX方法确定各站位的主要浮游植物类群，分析方法遵循Wright等人2010年发表的研究方案。 ### 达恩利角水华营养盐数据 溶解态营养盐浓度数据。样本由塔斯马尼亚州纽敦市圣约翰大道18号的初级产业、公园、水体与环境部完成分析。 ### 达恩利角走航数据VOYAGE_04_0_201112 科考补给船“极光南极号”在水华海域采集的原始走航数据。 ### 达恩利角走航数据地图 水华海域走航数据相关地图。 #### 数据质量说明 本次采样仅通过无污染海水采样管完成，采样口位于船舷下方4.8米处。但研究认为本次水华的主体分布于与海冰相关的表层海域，因此本数据集可能低估了真实的表层水华现象。 2018年10月10日：原始文件已重新格式化，以符合OBIS、GBFI、IPT及Biodiversity.AQ的数据集追踪标准，并生成了新的数据表"CapeDarnelyBloom_2012.csv"。新数据集包含以下字段：datasetID、occurrenceID、recordNumber、verbatimLatitude、verbatimLongitude、decimalLatitude、decimalLongitude、locationID、locality、eventRemark、eventDate、year、month、basisOfRecord、occurrenceStatus、organismQuantity、organismQuantityType、preparations、institutionCode、continent。经与世界海洋物种登记册（World Register of Marine Species，WoRMS）匹配后，本数据集提供了可鉴定到的最低分类阶元的物种信息。