Structure of D169A/E171A double mutant of chitinase Chit42 from Trichoderma harzianum complexed with chitintetraose.

Structure of D169A/E171A double mutant of chitinase Chit42 from Trichoderma harzianum complexed with chitintetraose. Descriptor: 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ACETATE ION, ... Authors: Jimenez-Ortega, E, Sanz-Aparicio, J. Deposit date: 2020-04-10 Release date: 2021-10-20 Last modified: 2024-01-24 Method: X-RAY DIFFRACTION (1.82 Å) Cite: Structural inspection and protein motions modelling of a fungal glycoside hydrolase family 18 chitinase by crystallography depicts a dynamic enzymatic mechanism Comput Struct Biotechnol J, 19, 2021

哈茨木霉（Trichoderma harzianum）来源的几丁质酶Chit42的D169A/E171A双突变体与几丁四糖（chitintetraose）复合的晶体结构。配体描述：2-乙酰胺基-2-脱氧-β-D-吡喃葡萄糖-(1-4)-2-乙酰胺基-2-脱氧-β-D-吡喃葡萄糖、2-乙酰胺基-2-脱氧-β-D-吡喃葡萄糖-(1-4)-2-乙酰胺基-2-脱氧-β-D-吡喃葡萄糖-(1-4)-2-乙酰胺基-2-脱氧-β-D-吡喃葡萄糖-(1-4)-2-乙酰胺基-2-脱氧-β-D-吡喃葡萄糖、乙酸根离子（ACETATE ION），…… 作者：Jimenez-Ortega, E、Sanz-Aparicio, J。提交日期：2020-04-10，发布日期：2021-10-20，末次修改日期：2024-01-24。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.82埃（Å）。引用文献：《通过晶体学对真菌糖苷水解酶家族18几丁质酶进行结构检视与蛋白质运动建模，揭示其动态酶促机制》，载于《计算与结构生物技术杂志（Computational and Structural Biotechnology Journal）》，2021年，第19卷