Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying G238S mutation in complex with boron-based inhibitor EC25

Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying G238S mutation in complex with boron-based inhibitor EC25 Descriptor: 1,2-ETHANEDIOL, 3-[(2R)-2-{[(2R)-2-amino-2-phenylacetyl]amino}-2-(dihydroxyboranyl)ethyl]benzoic acid, ACETATE ION, ... Authors: Dellus-Gur, E, Elias, M, Fraser, J.S, Tawfik, D.S. Deposit date: 2014-02-07 Release date: 2015-05-20 Last modified: 2024-10-16 Method: X-RAY DIFFRACTION (1.45 Å) Cite: Negative Epistasis and Evolvability in TEM-1 beta-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder. J. Mol. Biol., 427, 2015

携带G238S突变的稳定化TEM-1 β-内酰胺酶（beta-lactamase）变体v.13与硼基抑制剂EC25形成复合物的晶体结构。组分说明：该晶体复合物包含1,2-乙二醇、3-[(2R)-2-{[(2R)-2-氨基-2-苯乙酰基]氨基}-2-(二羟基硼基)乙基]苯甲酸、乙酸根离子等。作者：Dellus-Gur, E、Elias, M、Fraser, J.S、Tawfik, D.S。提交日期：2014-02-07，发布日期：2015-05-20，最后修改日期：2024-10-16。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.45埃。引用文献：《TEM-1 β-内酰胺酶中的负上位性与可进化性——酶构象自由度与无序性之间的细微界限》，《分子生物学杂志》，427卷，2015年。