Single-nuclei sequencing of the mouse hippocampus of dietary restricted mice

Dietary restriction (DR), reduced food intake while avoiding malnutrition, profoundly extends lifespan in most model and non-model organisms. Both chronic (i.e. life-long) and acute (i.e. late-onset) DR have been shown to improve cognitive performance in aged mice compared to animals with an unrestricted access to food (ad libitium feeding; AL). Yet so far, quantitative analyses of the molecular dynamics in the brain of DR fed animals have been limited. Here we performed single-nuclei sequencing (Nuc-seq) of whole hippocampus isolated from young (5 months) and old (24 months) AL fed animals, as well as old chronic DR (DR started at 3 months) and acute DR (aDR) mice. Overall design: Single-nuclei RNA transcriptomes of whole frozen hippocampus obtained from brains of young (5 months) and old (24 months) mice fed ad libitum (AL) as well as old mice that were dietary restricted from the age of 5 months (long-term DR; DR) or 20 months of age (acute DR; aDR) using 10x Genomics Drop-seq (v3.0). All animals were of the C3B6F1 hybrid strain.