Structurally Diverse PFAS Produce Unique Transcriptomic Changes Linked to Developmental Toxicity in Zebrafish. Structurally Diverse PFAS Produce Unique Transcriptomic Changes Linked to Developmental Toxicity in Zebrafish

Transcriptomic data for 48 hours post-fertilization (hpf) wildtype zebrafish developmentally exposed to 15 PFAS, and for 48, 72, and 96 hpf wildtype zebrafish developmentally exposed to nafion byproduct 2. Overall design: Three separate experiments: A (Control-A compared to PFAS 1, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13), B (Control-B compared to PFAS 2, 4, 14, 15), and C (Control-C compared to nafion byproduct 2 across developmental stages; s071-100). In all experiments, wildtype zebrafish embryos were statically exposed to PFAS beginning at 6 hours post-fertilization (hpf) until the sampling timepoint, when samples were extracted (Direct-zol RNA MiniPrep kit; Zymo) and processed through library prep (Lexogen QuantSeq 3' mRNA-seq FWD) and sequencing on an Illumina NextSeq 2000 (100 bp single end). For experiments A and B, all samples were collected at 48 hpf and each sample was split between two sequencing runs. Experimental Design for A consisted of controls and treated samples being collected across multiple days with intentional temporal variation, whereas B consisted of controls and treated samples all collected on a single day. For experiment C, samples were collected at 48, 72, and 96 hpf.