Machine Learning Identifies Signatures of Macrophage Reactivity and Tolerance

A continuum of macrophage polarization states is essential tissue homeostasis. We used machine learning approaches to identify universally relevant definition of macrophage polarization states and create a predictive framework for developing macrophage-targeted precision diagnostics and therapeutics. CCDC88A was identified as a key gene in the continuum state clusters that is essential for the tolerant polarization state. Murine peritoneal macrophage were isolated from macrophage specific CCDC88A (GIV) knockout mice and their littermate WT controls 4 days after thiglycollate injection (IP). Peritoneal macrophage were stimulated or not with 100ng/ml of LPS for 6h (n=3)