Binary Ti vectors pSiM24 produced higher plasmid DNA yields in Escherichia coli strain TB1 over pCAMBIA.

Three single colonies of each plasmid constructs were grown for 16 hrs in 25 ml LB media with 100 mg/L ampicillin (for pSiM24) or 50 mg/L kanamycin (for pCAMBIA) or 15 mg/L tetracycline (for pKM24KH). Plasmid DNA was purified using QIA Midiprep columns. DNA yields represent the average of three independent samples. Statistical analysis of the data was performed adopting one way ANOVA analysis (using GraphPad Prism version 5.01) and presented as the means ± S.D. A P value of less than 0.05 was considered significant indicated by different superscript letters.