Proteomics of Arabidopsis thaliana leaves after silencing of MKIP1 or uL4c

Arabidopsis thaliana (Arabidopsis) MATURASE K INTERACTING PROTEIN1 (MKIP1) is a plastid-localized, non-canonical member of the starch-branching enzyme family. Inducible silencing of MKIP1 by estradiol-treatment results in pale newly emerged leaves. Here we analysed the proteome of these leaves from two independent plant lines by LC-MS/MS using data-independent acquisition. Estradiol-treated wild-type (Col-0) plants and a line in which the plastidial ribosomal subunit uL4c was silenced served as controls. Data was analysed using DIA-NN via the FragPipe platform.