Physicochemical and biological properties of complexes 1 and 2.
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IC50 values were determined using an AlamarBlue Assay after 48 h of incubation with different concentrations of the complexes. The hydrolytic half life was determined using time resolved 1H-NMR. These hydrolysis experiments were conducted in a mixture of 95% [D8]THF and 4.8% D2O at 37°C. As an internal standard, 0.2% DMSO was added. Cell death was followed via double staining with propidium iodide and fluorescein isothiocyanate-labeled annexin V.
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2015-12-02
