Numeric data from: Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells

Stem and progenitor cell mitosis is essential for tissue development and homeostasis. How these cells ensure proper chromosome segregation, and thereby maintain mitotic fidelity, in the complex physiological environment of a living animal is poorly understood. Here we use in situ live-cell imaging of C. elegans germline stem and progenitor cells (GSPCs) to ask how the signaling environment influences stem and progenitor cell mitosis in vivo. Through a candidate screen we identify a new role for the insulin/IGF receptor (IGFR), daf-2, during GSPC mitosis. Mitosis is delayed in daf-2/IGFR mutants, and these delays require canonical, DAF-2/IGFR to DAF-16/FoxO insulin signaling, here acting cell non-autonomously from the soma. Interestingly, mitotic delays in daf-2/IGFR mutants depend on the spindle assembly checkpoint but are not accompanied by a loss of mitotic fidelity. Correspondingly, we show that caloric restriction, which delays GSPC mitosis and compromises mitotic fidelity, does not..., This dataset contains all numeric data reported in the paper \"Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells\". Details on data collection and processing can be found in the methods section of our paper. Measurement data include the duration of mitosis in germline stem cells in a range of genetic backgrounds and/or experimental conditions, the number of nuclei and mitotic cells per germline proliferative zone and germ line and germline stem cell fluorescent intensity measurements., , # Numeric data for manuscript: Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells

[https://doi.org/10.5061/dryad.sn02v6xfp](https://doi.org/10.5061/dryad.sn02v6xfp)

## Description of the data and file structure

These data were collected in support of the paper  \"Non-autonomous insulin signaling delays mitotic progression in *C. elegans *germline stem and progenitor cells which is available on bioRxiv ([https://doi.org/10.1101/2024.06.28.601188](https://doi.org/10.1101/2024.06.28.601188)) and in press at PLoS Genetics.

### Files and variables

#### File: Numeric\_Data\_for\_all\_figure\_panels.zip

**Description:** 

Data are organized by figure, with one folder per figure (e.g. Figure_1_RawData). Each folder contains one or more CSV files, with the file name indicating to which figure panel the numeric data belong.

CSV files were generated by exporting data tables from Matlab. The first row gives the column name. Missing val...