<p>Evaluation of WEPP’s unaccounted alleles and closest detected haplotype in the presence of novel haplotypes. WEPP’s sensitivity and precision in detecting novel haplotypes are assessed by (1) varying the novel haplotype’s proportion in the sample, and (2) adjusting the mutation distance between the novel haplotype and its closest sequence in the tree, defined by the ‘Node Removal Radius’. The columns represent: (i) the novel haplotype’s proportion, (ii) ‘Node Removal Radius’, (iii) the mutation distance between the novel haplotype and the closest haplotype detected by WEPP, (iv) the number of unaccounted alleles detected by WEPP, and (v) the number of alleles that reduce the distance between WEPP inferred haplotypes and the wastewater haplotypes.</p>

Evaluation of WEPP’s unaccounted alleles and closest detected haplotype in the presence of novel haplotypes. WEPP’s sensitivity and precision in detecting novel haplotypes are assessed by (1) varying the novel haplotype’s proportion in the sample, and (2) adjusting the mutation distance between the novel haplotype and its closest sequence in the tree, defined by the ‘Node Removal Radius’. The columns represent: (i) the novel haplotype’s proportion, (ii) ‘Node Removal Radius’, (iii) the mutation distance between the novel haplotype and the closest haplotype detected by WEPP, (iv) the number of unaccounted alleles detected by WEPP, and (v) the number of alleles that reduce the distance between WEPP inferred haplotypes and the wastewater haplotypes.