Phylogenomics and macroevolution of a florally diverse Neotropical plant clade

Hillieae is a group of ~30 florally diverse, Neotropical epiphyte species. Species richness peaks in southern Central America, and taxa display bat, hawkmoth, or hummingbird pollination syndromes. A phylogenetic framework is needed to understand floral and biogeographic evolution. We used target enrichment data to infer a species tree and a Bayesian time-calibrated tree including ~83% of the species in the group. We inferred ancestral biogeography and pollination syndromes, described species realized bioclimatic niches via a principal component analysis, and estimated significant niche shifts using Ornstein-Uhlenbeck models to understand how different abiotic and biotic variables have shaped Hillieae evolution. We estimated that Hillieae originated in southern Central America 19 Ma and that hawkmoth pollination is the ancestral character state. Multiple independent shifts in pollination syndrome, biogeographic distribution, and realized bioclimatic niche have occurred, though the biocli..., , , # Phylogenomics and macroevolution of a florally diverse Neotropical plant clade

Dataset DOI: [10.5061/dryad.4mw6m90k6](https://doi.org/10.5061/dryad.4mw6m90k6)

## Description of the data and file structure

This dataset includes cleaned and filtered multiple sequence alignments in nexus format, gene trees and species trees. Sequences were aligned using the program macse (version 2.07; Ranwez et al. 2018) under default settings, and the alignments were cleaned with the programs TAPER (version 1.1.1; Zhang et al. 2021), phyx (version 1.1; Brown et al. 2017), and trimAl (version 1.2; Capella-Gutiérrez et al. 2009), after being visually inspected with Geneious Prime (version 2021.1.1). Gene trees were inferred with RAxML-NG (Kozlov et al. 2019) under the GTR+G nucleotide evolution model. We inferred two species trees: a concatenated sequence tree using the program RAxML-NG, and a tree inferred under the multispecies coalescent model with weighted-ASTRAL (i.e., wASTRAL; Zhang and Mirarab ...,