Effects of PFKFB4 deletion on transcriptional reprogramming in breast cancer cells

To explore the mechanisms by which PFKFB4 promotes breast cancer metastasis, we CRISPR-Cas knock-out PFKFB4 in two breast cancer lines for RNA-seq to identify downstream target genes that are regulated by PFKFB4 Overall design: Single-end mRNA sequencing was performed from wild-type (WT) or PFKFB4-knock-out (PFKFB4-KO) mouse EMT6 and human MDA-MB-231 breast cancer cells