In this paper, we present an objective method for locally quantifying proteins in blood brain barrier (BBB) vasculature using standard immunohistochemistry (IHC) techniques and bright-field microscopy
Known quantities of a His-tagged purified protein were blotted with His-ABCH2 and ABCH2 membrane preparations (S11 Fig). ImageJ was used for pairwise band density analysis. The density values and the
Protein intensities measured, with median subtraction for normalization, combined file for all species. Input file used in the online database at atlas.cardiacproteomics.com. (XLSX)
