Expression data from Sos1 and Sos2-depleted (individually or combined) mouse primary fibroblasts

Sos1 and Sos2 are guanine-nucleotide exchange factors for Ras and Rac small GTPases, which are involved in a wide range of cellular responses including proliferation and migration. We have previously shown that Sos1 and Sos2 have different effects on cell migration, but the underlying mechanisms are not clear. Here, we examined the role of Sos1 and Sos2 in the overall transcriptional profile of mouse primary fibroblasts in order to unveil the specific functions of these Ras/Rac activators in regulating migration. We used microarrays to detail the impact of Sos1/2 depletion (individual or combined) in the transcriptional signature of mouse primary keratinocytes Mouse primary fibroblasts (WT, Sos1KO, Sos2KO and Sos1/2DKO) at actively growing conditions 4-hydroxytamoxifen-treated for 12 days were selected for RNA extraction and hybridization on Affymetrix microarrays. RNA was isolated and hybridated following manufacturer's recommendations.