Genome sequencing with short reads

To test methods for assembly of very short reads, five microbial genomes were selected which have a finished reference sequence available. Rhodobacter sphaeroides 2.4.1 was one of these five microbial genomes. At least two libraries (short fragment and jumping) were generated and sequenced using Illumina. Genomic DNA from Baylor. Two different genome assemblies are part of this project. The assembly in AKBU00000000 is from Illumina reads and contains 2 complete chromosomes, 3 complete plasmids and 2 incomplete plasmids that share long perfect overlaps, so are present as 6 contigs. The assembly in AKVW00000000 is from sequences in BioProject PRJNA56 plus reads from a different isolate of the strain. It contains 2 complete chromosomes and 5 complete plasmids, but the plasmid sequences differ from those in the genome assembled in BioProject PRJNA56.