Additional file 4 of Detection and discovery of plant viruses in soybean by metagenomic sequencing

Additional file 4 Figure S4. Plant viruses confirmed by Sanger sequencing in field grown soybean plants. Reverse transcription-polymerase chain reaction (RT-PCR) was performed in 78 field samples. The PCR products were visualized in a 1% agarose gel electrophoresis containing SYBR Safe DNA gel stain. The PCR products were cleaned using ExoSAP-IT PCR product cleanup reagent and sequenced on Sanger sequencing platform. Primer sequences were designed using Primer3 plus from assembled contigs and the primers were used in this study.