Peer review

To perform targeted metabolomic analysis of medium- and long-chain fatty acids based on GC-MS, we first cultured the MAT-1 and sscyp86Δ-1 strains overnight in YePS solution at 200 rpm and 28℃. We then centrifuged the cultures, washed the collected cells once with sterilized ddH2O, resuspended the cells, and diluted the cell suspension to an OD600 of 1.0. Equal volumes of the resulting cell suspension were spread onto YePS agar. The plates were incubated at 28℃ for 42 h, and then the yeast colonies of S. scitamineum were scraped off and sent to Shanghai Applied Protein Technology Co., Ltd. for metabolomic analysis.