Data for: Mechanism of nitric oxide and acid-sensing ion channel 1a modulation of panic-like behaviour in the dorsal periaqueductal grey

Fig. 1. Photograph of the study apparatus. Fig. 2. Effects of SNAP infusion (10 or 20 nmol/0.2 μl) in the dPAG on ethological and spatiotemporal measures. (A) Freezing time; (B) stretched-attend posture (SAP); (C) time in contact with the grid wall; and (D) time in the protected area. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (* P < 0.05, ** P < 0.01, and *** P < 0.001 compared with the control group injected with vehicle. n = 8-10 mice/group). Fig. 3. Effects of L-NAME infusion (100 or 300 nmol/0.2 μl) in the dPAG on ethological and spatiotemporal measures. (A) Freezing time; (B) stretched-attend posture (SAP); (C) time in contact with the grid wall; and (D) time in the protected area. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (** P < 0.01 and *** P < 0.001 compared with the control group injected with vehicle. n = 8-10 mice/group). Fig. 4. Effects of PcTx-1 infusion (20 or 50 ng/0.2 μl) in the dPAG on ethological and spatiotemporal measures. (A) Freezing time; (B) stretched-attend posture (SAP); (C) time in contact with the grid wall; and (D) time in the protected area. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (* P < 0.05 and ** P < 0.01 compared with the control group injected with vehicle. n = 8-10 mice/group). Fig. 5. Effects of SNAP treatment on ASIC1a, p-CaMKIIα, and CaM expression levels in the dPAG. Representative Western blot image (A) and results of quantification of the expression levels of ASIC1a (B), p-CaMKIIα (C) and CaM (D) in the vehicle control (0) and SNAP treatment groups. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (*P < 0.05 and **P < 0.01 compared with the control group). Fig. 6. Effects of L-NAME treatment on ASIC1a, p-CaMKIIα, and CaM expression levels in the dPAG. Representative Western blot image (A) and results of quantification of the expression levels of ASIC1a (B), p-CaMKIIα (C) and CaM (D) in the vehicle control (0) and SNAP treatment groups. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (*P < 0.05 and **P < 0.01 compared with the control group). Fig. 7. Effects of PcTx-1 treatment on p-CaMKIIα and CaM expression levels in the dPAG. Representative Western blot image (A) and analysis to quantify the expression levels of p-CaMKIIα (B) and CaM (C) in the vehicle control (0) and SNAP treatment groups. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (*P < 0.05 compared with the control group).

图1. 实验装置实拍图。
图2. 背侧导水管周围灰质（dorsal periaqueductal gray, dPAG）内输注SNAP（10或20 nmol/0.2 μl）对行为学及时空测量指标的影响。(A) 冻结时间；(B) 伸展警戒姿势（stretched-attend posture, SAP）；(C) 接触网格壁的时长；(D) 防护区域停留时长。通过单因素方差分析（one-way ANOVA）与Dunnett多重比较法确定给药组与溶剂对照组的显著性差异（与赋形剂注射的对照组相比：* P < 0.05，** P < 0.01，*** P < 0.001；每组n=8~10只小鼠）。
图3. 背侧导水管周围灰质（dPAG）内输注L-NAME（100或300 nmol/0.2 μl）对行为学及时空测量指标的影响。(A) 冻结时间；(B) 伸展警戒姿势（SAP）；(C) 接触网格壁的时长；(D) 防护区域停留时长。通过单因素方差分析与Dunnett多重比较法确定给药组与溶剂对照组的显著性差异（与赋形剂注射的对照组相比：** P < 0.01，*** P < 0.001；每组n=8~10只小鼠）。
图4. 背侧导水管周围灰质（dPAG）内输注PcTx-1（20或50 ng/0.2 μl）对行为学及时空测量指标的影响。(A) 冻结时间；(B) 伸展警戒姿势（SAP）；(C) 接触网格壁的时长；(D) 防护区域停留时长。通过单因素方差分析与Dunnett多重比较法确定给药组与溶剂对照组的显著性差异（与赋形剂注射的对照组相比：* P < 0.05，** P < 0.01；每组n=8~10只小鼠）。
图5. SNAP给药对背侧导水管周围灰质（dPAG）内酸敏感离子通道1a（ASIC1a）、磷酸化钙调蛋白激酶IIα（p-CaMKIIα）及钙调蛋白（CaM）表达水平的影响。(A) 代表性蛋白质免疫印迹（Western blot）图像；(B)(C)(D) 分别为溶剂对照（0）组与SNAP给药组中ASIC1a、p-CaMKIIα及CaM的表达水平定量结果。通过单因素方差分析与Dunnett多重比较法确定给药组与溶剂对照组的显著性差异（与对照组相比：* P < 0.05，** P < 0.01）。
图6. L-NAME给药对背侧导水管周围灰质（dPAG）内ASIC1a、p-CaMKIIα及CaM表达水平的影响。(A) 代表性蛋白质免疫印迹图像；(B)(C)(D) 分别为溶剂对照（0）组与L-NAME给药组中ASIC1a、p-CaMKIIα及CaM的表达水平定量结果。通过单因素方差分析与Dunnett多重比较法确定给药组与溶剂对照组的显著性差异（与对照组相比：* P < 0.05，** P < 0.01）。
图7. PcTx-1给药对背侧导水管周围灰质（dPAG）内p-CaMKIIα及CaM表达水平的影响。(A) 代表性蛋白质免疫印迹图像；(B)(C) 分别为溶剂对照（0）组与PcTx-1给药组中p-CaMKIIα及CaM的表达水平定量结果。通过单因素方差分析与Dunnett多重比较法确定给药组与溶剂对照组的显著性差异（与对照组相比：* P < 0.05）。