IIb-RAD-seq coupled with random forest classification indicates regional population structuring and sex-specific differentiation in salmon lice (Lepeophtheirus salmonis)
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The aquaculture industry has been dealing with salmon lice problems forming serious threats to salmonid farming. Several treatment approaches have been used to control the parasite. Treatment effectiveness must be optimized, and the systematic genetic differences between sub-populations must be studied to monitor louse species and enhance targeted control measures. We have used IIb-RAD sequencing in tandem with a random forest classification algorithm to detect the regional genetic structure of the Norwegian salmon lice and identify important markers for sex differentiation of this species. We identified 19428 single nucleotide polymorphisms (SNPs) from 95 individuals of salmon lice. These SNPs, however, were not able to distinguish differential structure of lice populations. Using the random forest algorithm, we selected 91 SNPs important for geographical classification and 14 SNPs important for sex classification. The geographically important SNP data substantially improved the geneti...
水产养殖业长期面临鲑鱼虱（salmon lice）问题的困扰，该寄生虫已对鲑科鱼类养殖构成严重威胁。目前虽已采用多种防治手段管控该寄生虫，但仍需优化防治效果，同时需深入研究亚种群间的系统性遗传差异，以实现虱类物种监测并强化针对性防控措施。本研究采用IIb-RAD测序（IIb-RAD sequencing）结合随机森林分类算法，对挪威鲑鱼虱的区域遗传结构进行检测，并筛选出该物种性别分化的关键分子标记。研究团队从95尾鲑鱼虱个体中共鉴定出19428个单核苷酸多态性（single nucleotide polymorphisms, SNPs）位点。然而，上述SNPs无法有效区分虱类种群的遗传分化结构。通过随机森林算法，我们筛选出91个与地理分类相关的关键SNPs，以及14个与性别分类相关的关键SNPs。具备地理区分能力的SNPs数据显著提升了遗传...
