Products differentially secreted from perigonadal adipose tissue of overfed mice.

Male C57BL/6J mice were implanted with intragastric catheters that allowed direct infusion of liquid into the stomach. A control group of mice received saline through the catheter and was given free access to high-fat diet (Research Diets D12492i, 60% calories from fat) over a span of months, leading to “ad libitum obesity” (ALO). Another group of mice received a hypercaloric liquid diet through the catheter, leading to an acute-onset, “overfeeding-induced obesity” (OIO). OIO mice were also given free access to high-fat diet but did not consume it while receiving hypercaloric intragastric infusions. After 12 days of infusions, the average weights of the two groups were equal. All mice were fasted for 24 hours, after which they were sacrificed and perigonadal adipose tissue (PGAT) was dissected out. PGAT was cultured ex vivo for 6 hours, after which conditioned media was collected and submitted for proteomic profiling. All mice were age-matched and 16 weeks old at time of sacrifice. “HFD” is also used to refer to the ALO condition, while “OID” is also used to refer to the OIO condition.