Estimated divergence values between the guppy, Poecilia reticulata, and other species, including M. picta, M. parae, Xiphophorus maculatus (the platyfish) and Gambusia affinis, for multiple genes.

The top part shows synonymous site divergence (Ks), non-synonymous site divergence (Ka) and divergence estimated using all site types, based on nuclear genes from multiple guppy automosomes (column F, labelled “LG” for linkage group). Ka/Ks values are also shown; most genes have values suggesting selective constraint, although two of the 22 genes (ENSPRET00000002334.1 on LG2, and ENSPRET00000011682 on LG4, appear to be evolving neutrally. Chromosome 2 of the guppy is a fused chromosome, and the genes analysed were from the arm that carries homologues of genes on X. maculatus LG7. The lower part of column Q shows Ks values between M. picta and M. parae, for 7 genes whose sequences were obtained by Pollux et al. (2014 The evolution of the placenta drives a shift in sexual selection in livebearing fish. Nature 513: 233–236. doi: 10.1038/nature13451). The Ks estimates are plotted in Fig 3. (XLSX)