Aging Gut Microbiome: Butyrate Loss and Associated Functional Decline in a Cypriot Non-Adenoma Cohort

This dataset contains the normalized 16S rRNA OTU data and corresponding sample metadata used in the associated study. Data generation: OTUs were generated from raw sequencing reads using the BaseSpace (Illumina) 16S Metagenomics app v1.1.3 with the RefSeq RDP 16S v3 reference database. The resulting OTU table was rarefied to even sequencing depth in R prior to downstream analysis. Analyses: The deposited data were used in R for alpha and beta diversity estimation (vegan), post-hoc statistical testing (agricolae), and correlation analyses including partial correlations adjusted for covariates (ppcor). Full methodological detail is provided in the associated publication. Code availability: Custom analysis scripts are available from the corresponding author upon reasonable request.