Data from: Rapid detection of cocaine using aptamer-based biosensor on an evanescent wave fiber platform

The rapid detection of cocaine has received considerable attention because of the instantaneous and adverse effects of cocaine overdose on human health. Aptamer-based biosensors for cocaine detection have been well established for research and application. However, reducing the analytic duration without deteriorating the sensitivity still remains as a challenge. Here, we proposed an aptamer-based evanescent wave fiber (EWF) biosensor to rapidly detect cocaine in a wide working range. At first, the aptamers were conjugated to complementary DNA with fluorescence tag and such conjugants were then immobilized on magnetic beads. After cocaine was introduced to compete against the aptamer-DNA conjugants, the released DNA in supernatant was detected on the EWF platform. The dynamic curves of EWF signals could be interpreted by the first order kinetics and saturation model. The semi-log calibration curve covered a working range of 10-5000 μM of cocaine, and the limit of detection was approximately 10.5 μM. The duration of the full procedure was 990 s (16.5 min), and the detection interval was 390 s (6.5 min). The specified detection of cocaine was confirmed from four typical pharmaceutic agents. The analysis was repeated for 50 cycles without significant loss of sensitivity. Therefore, the aptamer-based EWF biosensor is a feasible solution to rapidly detect cocaine.由于可卡因过量会对人体健康产生即时且有害的影响，快速检测可卡因一直受到广泛关注。用于可卡因检测的适配体（aptamer）生物传感器已在研究与应用中得到充分验证。然而，在不降低检测灵敏度的前提下缩短分析时长仍是一项挑战。本研究提出一种基于适配体的倏逝波光纤（evanescent wave fiber, EWF）生物传感器，可在宽工作范围内快速检测可卡因。首先，将适配体与带有荧光标记的互补DNA结合，随后将此类结合物固定于磁珠表面。当加入可卡因与适配体-DNA结合物竞争结合后，上清液中释放的DNA可在EWF平台上完成检测。EWF信号的动态曲线可通过一级动力学模型与饱和模型进行解析。该传感器的半对数校准曲线覆盖10~5000 μM的可卡因检测范围，检出限约为10.5 μM。完整检测流程耗时990秒（即16.5分钟），单次检测间隔为390秒（即6.5分钟）。通过四种典型药剂验证了该传感器对可卡因的特异性检测能力。该检测流程重复50个循环后，灵敏度未出现显著下降。综上，基于适配体的EWF生物传感器是实现可卡因快速检测的可行方案。