HCC38_Human_Cancer_Cell_Line_Study__Single_Cell__

The HCC38 breast cancer cell line has previously been used as a model to explore the rate at which mutations occur during the cell cycle. Here we are assessing the applicability of a new whole genome amplification, called MALBAC, which offers improved genome coverage in single cell genomic sequencing. In this study, MALBAC amplification will be applied to single cells, related by a single cell division. The MALBAC amplification method is, in part, a PCR based method which generates a product of ~300-3,000 bp with a yield of 300-3,000 ng per single cell, depending on the condition and reagents used. In the first instance, this study aims to compare the data generated from these cells with data generated previously using alternative methods for Whole Genome Amplification including Multiple Displacement Amplification and PCR based methods (PicoPlex).