Phenotypic and genotypic analysis of drug resistance in M. tuberculosis isolates in Gansu, China

Tuberculosis has posed a serious threat to human health. It is imperative to investigate the geographic prevalence of tuberculosis and medication resistance, as this information is essential for informing strategies for its prevention and treatment. Drug resistance was identified using a proportion method. Drug-resistant genes and pathways were predicted using whole genome sequencing. The drug resistance range of bedaquiline was identified using the microporous plate two-fold dilution method, and drug resistance genes were studied using sequencing. The study revealed that 19.99% of the tuberculosis cases had multidrug resistance. The genes of M. tuberculosis are predominantly involved in the synthesis of ABC transporters, two-component systems, and bacterial secretion systems, as well as in energy production and conversion, and lipid transport and metabolism. The genes encode for 82.45% of carbohydrate-related enzymes such as glycoside hydrolases, glycosyl transferases, and carbohydrate..., , , # Phenotypic and genotypic analysis of drug resistance in M. tuberculosis isolates in Gansu, China
([https://doi.org/10.5061/dryad.r4xgxd2np](https://doi.org/10.5061/dryad.r4xgxd2np)

Complete genome sequence of Mycobacterium tuberculosis，to identify resistance loci, resistance genes and pathways were predicted using whole-genome sequencing, and to identify mutation sites, we performed sequencing.

\## wgs-tb-1: whole genome sequence

\## Sharing/Access information

Data was derived from the following sources:

(Beijing Biomarker Technologies Co., LTD)

\## Code/Software

Any sequence analysis software can be run.