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Single-cell RNA-seq of mouse cells from the dorsal cerebral cortex during its formation

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干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=a6a7c7250883efc8a90ff667fea3489b
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Dorsal cortices from embryonic day (E10.5) to postnatal day 3 (P3) were micro-dissected and dissociated into single cell suspensions using Papain and Ovo-mucoid mix. Cells were washed with L15 medium and FAC-sorted for GFP positive NSCs using FACSariaIII (BD Biosciences) derived from Hes5::GFP transgenic embryos for NSCs and Tbr2::GFP transgenic embryos for BPs and NBNs.
提供机构:
University of Basel
创建时间:
2022-02-20
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