The therapeutic mechanism of Fuzheng-Qushi formula on lung injury in mice revealed by transcriptomics

FZQS treatment significantly ameliorated lung histopathological damage, decreased pro-inflammatory cytokine levels and increased anti-inflammatory cytokine levels. The level of M1 macrophages in the peripheral blood of lung-injured mice decreased, the level of M2 macrophages increased, and activated neutrophils were inhibited. Importantly, transcriptomic analysis showed that FZQS downregulated macrophage and neutrophil activation, migration and adhesion pathways by reversing 51 DEGs, which was further confirmed by RT-qPCR and Western blot.