Characterization of human iPSC-derived ocular surface epithelial cell sheets depending on PAX6 gene expression levels

PAX6-EGFP, TP63-tdTomato reporter iPSCs were cultured using the self-formed ectodermal autonomous multi-zone (SEAM) method (Hayashi et al, Nature 2016) for 10 weeks to generate ocular surface epithelial cell sheets. These epithelial cells were isolated and defined as EGFP-/tdTomato+ (P1), EGFPlow/tdTomato+ (P2), EGFPmid/tdTomato+ (P3), and EGFPhigh/tdTomato+ (P4).