Development of an LC–MS/MS assay forquantification of intact INSL3 in rat plasma: supplementary data
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https://figshare.com/articles/dataset/Development_of_an_LC_MS_MS_assay_forquantification_of_intact_INSL3_in_rat_plasma_supplementary_data/24101313
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资源简介：
Background: Relatively large disulfide-linked polypeptides can serve as signaling molecules for a diverse
array of biological processes and may be studied in animal models to investigate their function in vivo.
The aim of this work was to develop an LC–MS/MS assay tomeasure a model peptide, INSL3, in rat plasma.
Results: A dual enrichment strategy incorporating both protein precipitation and solid phase extraction
was utilized to isolate INSL3 from rat plasma, followed by targeted LC–MS/MS detection. The method was
able to measure full-length INSL3 (6.1 kDa) down to 0.2 ng/ml with acceptable accuracy and precision.
Conclusion: The final assay was applied to support an exploratory pharmacokinetic study to evaluate
steady-state concentrations of dosed INSL3 in rat plasma.
创建时间：
2023-09-07
