Additional file 1: of Transcriptome analysis and identification of genes associated with ω-3 fatty acid biosynthesis in Perilla frutescens (L.) var. frutescens

Table S1. Fatty acid composition of perilla developing seeds and leaves. All data are averages of three measurements ± SE. Table S2. Statistical summary of de novo transcriptome assembly and annotation. Table S3. Annotation of 54,079 perilla representative transcripts and their expression in developing seeds and leaf. Transcripts represents as Locus. Raw read count and normalized read count were represented as expression level for each developing seeds and leaves samples. Table S4. Nucleotide sequences of 54,079 perilla representative transcripts in FASTA format. Table S5. Gene ontology analysis of DEG cluster 3, 4, 5 and 10 transcripts of perilla. Table S6. List of gene products for the top 50 DEGs in Cluster 3, 4, 5 and 10. Table S7. Number of identified genes involved in acyl-lipid metabolism of perilla transcriptome. Table S8. List of expressed genes involved in fatty acid and TAG biosynthesis in perilla. Table S9. Information of perilla gene primers (5′ → 3′) used in the qPCR analysis and cDNA cloning. (ZIP 27440 kb)

附表S1：紫苏发育种子与叶片的脂肪酸组成。所有数据均为三次测量的平均值±标准误（Standard Error，SE）。附表S2：紫苏从头转录组组装与注释的统计汇总。附表S3：54079条紫苏代表性转录本的注释信息，及其在发育种子与叶片中的表达情况。转录本以基因座（Locus）标识；各发育种子和叶片样本的表达水平以原始读段计数（raw read count）与标准化读段计数（normalized read count）表示。附表S4：以FASTA格式存储的54079条紫苏代表性转录本的核苷酸序列。附表S5：紫苏差异表达基因（Differentially Expressed Genes，DEG）聚类簇3、4、5与10的转录本的基因本体（Gene Ontology，GO）分析结果。附表S6：聚类簇3、4、5与10中排名前50的差异表达基因的基因产物列表。附表S7：紫苏转录组中参与酰基脂代谢的已鉴定基因数量统计。附表S8：紫苏中参与脂肪酸与三酰甘油（Triacylglycerol，TAG）生物合成的表达基因列表。附表S9：本研究实时定量PCR（quantitative real-time polymerase chain reaction，qPCR）分析与cDNA克隆所用的紫苏基因引物信息（5′→3′）。（压缩包大小：27440 kb）