High-throughput single-cell transcriptome profiling of plant cell types. High-throughput single-cell transcriptome profiling of plant cell types

Single-cell transcriptome analysis of heterogeneous tissues can provide high-resolution windows into the spatiotemporal dynamics of developmental processes and environmental responses. Here we use the high-hroughput Drop-seq approach to profile the transcriptomes of over 12,000 individual cells from the Arabidopsis root. We identify marker genes for a diversity of cell types, capture cell-type frequency and gene expression alterations in response to sucrose, and illuminate the transcriptome changes that occur across endodermis development. Overall design: Single-cell transcriptomes were captured from over 12,000 cells, across 10 drop-seq runs (6 independent protoplast preparations)