Gene expression of basal alonal, luminal alonal, basal and luminal aggregates during collective migration

Breast epithelium has two types of epithelial cells: basal and luminal. We found that basal cells promote the leadership of luminal cells during duct development and collective migration, but it is not clear how. Here, through this sequencing, we found that basal cells promote oxidative phosphorylation of luminal cells through EFNA3, allowing it to play a role in migration. Overall design: To investigate the role of basal and luminal cells in collective migration at the mRNA level, we separately aggregated basal cells, luminal cells, and a 50% mixture of both basal and luminal cells. The samples were collected after migrating for 56 hours under the induction of FGF10 beads.