Single-cell transcriptome of PBMC response to Omicron breakthrough infection

To gain a deeper understanding of Omicron waves in the context of vaccination, we performed scRNA-seq together with single-cell V(D)J sequencing using PBMCs from nine Omicron breakthrough infection patients and six vaccinees to identify the possible cellular and molecular response mechanisms after breakthrough infection. We recruited 9 laboratory-confirmed COVID-19 patients and 6 booster vaccinees to form the droplet-based scRNA-seq (10X Genomics). Their ages ranged from 23 to 57 years old, and 7 of them were male. The EDTA anticoagulant peripheral blood from patients and vaccinees was collected in acute phase (within 7 days post symptom onset) and over 1 month after the last vaccination, respectively.