Protein kinase constructs and properties.

Protein kinase constructs and properties. Overview of the constructsused in this study, indicating protein name and Uniprot-ID (U-ID), protein domain contents and the corresponding amino acids of the relative Uniprot sequences (FL: full length; CD: catalytic domain). Also indicated is Km for ATP that we determined for each construct, the concentration of ATP present in the assay for each kinase, the amount of enzyme used and the assay substrate peptide sequence (5F: 5-FAM or 5- Fluorescein AMidite tag).