Genome Sequencing of marine-derived Basidiomycete Peniophora sp. CBMAI 1063. Genome Sequencing of marine-derived Basidiomycete Peniophora sp. CBMAI 1063

The marine environment has enormous microbial diversity that remains unexplored. In particular, filamentous fungi can be easily isolated from the inner tissue of marine sponges and have been gaining attention since it is a reservoir of novel enzymes with biotechnological applications. In this work, we report the genome sequencing and annotation of the marine-derived Basidiomycete Peniophora sp. CBMAI 1063, as well as, secretome analyses of the fungus grown under an optimized condition for enzyme production. The resulting draft ge-nome assembly of Peniophora sp. CBMAI 1063 is 46,982,497 bp in length and encodes 17,714 protein-coding genes with 11,802 clusters of orthologous. The comparison of the predicted genes against the Carbohydrate-Active Enzymes (CAZy) database identified a total of 310 proteins related to lignocellulose degradation and cell wall modifications. Secretome analyses revealed that 48% of the identified proteins are related to lignocellulose degradation, especially lignin degradation and modification. Aiming to disclose new oxidative enzymes with biocatalytic activities for lignocellulose deconstruction, the major laccase secreted by the fungus was purified and its effects on lignin degradation were investigated. The omics analysis collectively show that Peniophora sp. CBMAI 1063 possesses a complete and versatile ligninolytic enzymatic spectrum with biotechnological potential to be explored, and the major secreted laccase can be applied for deconstruction of lignocellulose material into fuels and chemicals.