ManifoldEM: ESPER Data and Code Repository

This repository contains code to apply the ESPER method to quasi-continuum models of biomolecules exhibiting multiple degrees of freedom, as described in Seitz et al. (2022, IEEE TCI). As inputs into ESPER, detailed instructions are also provided for generating custom synthetic datasets with increasing complexity to mirror known cryo-EM image attributes. As well, this repository contains the complete Hsp90 dataset analyzed in the 2022 study, generated along a great circle with experimentally-relevant occupancies, signal-to-noise ratio, and contrast transfer function, and includes all corresponding ESPER outputs.

本存储库包含了将 ESPER 方法应用于具有多个自由度的生物大分子准连续模型的相关代码，如 Seitz 等人所述（2022，IEEE TCI）。作为 ESPER 的输入，还提供了详细指南，用于生成具有递增复杂度的定制合成数据集，以模拟已知的冷冻电子显微镜（cryo-EM）图像特征。此外，本存储库还包含了2022年研究中分析的完整Hsp90数据集，该数据集沿大圆生成，具有实验相关的占有率、信噪比和对比度传递函数，并包括所有相应的 ESPER 输出。