A specialized SKI complex assists the cytoplasmic RNA exosome in the absence of direct association with ribosomes

The Ski2-Ski3-Ski8 (SKI) complex assists the RNA exosome during the 3 0 -5 0 degradation of cytoplasmic transcripts. Previous reports showed that the SKI complex is involved in the 3 0 -5 0 degradation of mRNAs, including 3 0 untranslated regions (UTRs) and devoid of ribosomes. Paradoxically, we recently showed that the SKI complex directly interacts with ribosomes during the co-translational mRNA decay and that this interaction is necessary for its RNA degradation promoting activity. Here, we characterised a new SKI-associated factor, Ska1, that associates with a subpopulation of the SKI complex. We showed that Ska1 is specifically involved in the degradation of long 3 0 UTR-containing mRNAs, poorly translated mRNAs as well as other RNA regions not associated with ribosomes, such as cytoplasmic lncRNAs. We further show that the overexpression of SKA1 antagonises the SKI-ribosome association. We propose that the Ska1-SKI complex assists the cytoplasmic exosome in the absence of direct association of the SKI complex with ribosomes.