Transcriptional profiling by single cell-RNA sequencing of HARA cells according to Calbindin and HERVH-LTR7 activity

The purpose of this study to examine the effect of Calbindin expression, driven by an upstream HERVH LTR (human endogenous retrovirus H long terminal repeat), in HARA squamous lung cancer cells. Parental HARA cells, Calbindin-deficient HARA 3D5 cells, HARA.LTR7-GFP+ and HARA.LTR7-GFP- cells were compared and were processed as a single batch, using the 10× Genomics Chromium platform.