Binding profile of Halobacterium salinarum histone [ChIP-Seq]

Unlike the characterised histone proteins of a number of methanogenic and themophilic archaea, previous research indicated that HpyA, the sole histone encoded in the model halophile Halobacterium salinarum, is not involved in DNA packaging. Instead, it was found to have widespread but subtle effects on gene expression, and affect cell morphology. Here we report that HpyA is important for growth in reduced salinity and regulates gene expression of ion uptake and nucleotide metabolism pathways in a salt-dependent manner. Analysis of the genome-wide binding pattern of HpyA reveals that its binding is dependent on external salt concentration as well as growth phase. Combining the transcriptomic and ChIP-Seq data suggests a role for HpyA as a regulator of the expression of ion uptake genes as well as a co-regulator of other genes of diverse functions in a salt-dependent manner. In this GEO series , we use ChIP-Seq to study the genome-wide binding of HpyA-HA in optimal (4.2M NaCl) and reduced (3.4M) in exponential and stationary phase, using HA-tag-allone strains as a control Examination of HpyA binding sites in the halophilic archaeon Halobacterium salinarum in mid-logarithmic and stationary phases of growth, in optimal (4.2M NaCl) and reduced (3.4M NaCl) salt concentrations.