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Data from: Endoscopic liquid biopsies of gastric fluid in a large human patient cohort reveal DNA content as a candidate tumor biomarker in gastric cancer

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DataCite Commons2026-04-01 更新2026-04-25 收录
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https://datadryad.org/dataset/doi:10.5061/dryad.bzkh189qz
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Patient data: collected from medical records.  DNA extraction and quantification: DNA was extracted from gastric fluids that are routinely removed to allow better examination of the mucosa. These fluids were collected in sterile plastic containers attached to the endoscope suction channel and kept on ice until pH measurement and neutralization. Samples were divided in aliquots and kept frozen at -20<sup>o</sup>C (each aliquot was thawed just once), until DNA was extracted from an equal volume of gastric fluid (800 µl) by proteinase-K digestion, followed by phenol-chloroform extraction and ethanol DNA precipitation. DNA was subsequently resuspended in 100 µl of sterile nuclease-free double-deionized water and quantified by Qubit fluorometry (Thermo Fisher).
提供机构:
Dryad
创建时间:
2026-04-01
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