Effect of boosting in mice after a long period of non-exposure.

Evolution of the anti-G. m. morsitans saliva IgG responses (A) and the anti-rTsal1 IgG repsonses (B) was evaluated in mice (n = 6/group) originally exposed to the bites of a single or 10 flies and re-exposed to a single tsetse fly bite after a 1-year period of non-exposure (indicated by an arrow). Controls are the naive mice exposed to a single bite as depicted in Figure 1. The original control animals (n = 3) were not exposed and served as negative controls (dotted line). Presented data are mean ΔO.D.450 nm values with the 95% CI obtained with a 1∶1600 dilution of the individual plasmas.