CIL:38871, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

In Euplotes a replication band of the macronucleus has larger heterochromatin masses on one side of the band after DNA replication has occurred. The replication band moves in the direction of lighter staining in the macronucleus. Vacuoles containing concretion bodies are observed. TEM taken on 8/03/67 by R. Allen with Philips 200 operating at 60kV. Neg. 7,200X. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).在游仆虫（Euplotes）中，DNA复制完成后，其大核（macronucleus）的复制带（replication band）一侧会存在更大的异染色质（heterochromatin）团块。该复制带会朝着大核内染色较浅的区域移动。可观察到含有凝结体（concretion bodies）的液泡。本图像由R. Allen于1967年3月8日使用加速电压为60kV的飞利浦200型透射电子显微镜（Transmission Electron Microscopy, TEM）拍摄，原始负片放大倍数为7200倍。样品采用标准戊二醛固定、四氧化锇（osmium tetroxide）后固定，经酒精脱水后以环氧树脂（epoxy resin）包埋，使用超薄切片机（microtome）制备厚度约75nm的超薄切片。原始胶片通过尼康Coolscan 9000ED扫描仪进行数字化扫描。本图像适用于定量分析。更多详细信息可访问：http://www5.pbrc.hawaii.edu/allen/。