The synergestic effect of adaptive laboratory evolution on H2O2 and galactose on the gene expression profiles of genetically modified, taxadiene-producing Saccharomyces cerevisiae before and during the oxidative stress re-exposure

We attempted to improve the resistance of taxadiene-producing yeast strain to oxidative stress to develop a more robust yeast cell factory for improved Taxol® drug oxyenated taxanes precursors production from taxadiene. To this end, we evolved a yeast strain on H2O2-containing defined growth medium, supplemented with galactose as carbon source to induce the heterologous taxadiene biosynthesis pathway genes in that strain. The oxidative stress re-induction effect on the expression profiles of the superior evolved yeast strain (E_LRS5) was then studied before (steady state I) and during its continous use (steady state II) in galactose-limited chemostats, in parallel with the parent strain (LRS5). Comparative gene expression profiling analysis of RNA-seq data for parent (LRS5) and H2O2 and galactose-evolved taxadiene-producing S. cerevisiae (E_LRS5) strains before (steady state I) and during the exposure to H2O2 (steady state II) in galactose-limited chemostats