donkey astrovirus DAstV-1
收藏科学数据银行2023-03-30 更新2026-04-23 收录
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资源简介：
we collected intestinal samples from six deceased donkey foals, stored them at -80 ℃, and subsequently transported them to our laboratory for pathogen detection with virome. The samples were homogenized with steel beads and total RNA was extracted using an Omega viral DNA/RNA kit (Omega, USA). Next, a mixture of six nucleic acid samples was tested using nextgeneration sequencing. A sequencing library was constructed using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB, USA). The library was sequenced on an Illumina Novaseq 6000 (PE150) sequencing platform (San Diego, CA, USA).
提供机构：
Beijing University of Chemical Technology
创建时间：
2023-03-29
