Structure of the PRO-PRO endopeptidase (PPEP-3) E153A Y189F in complex with substrate peptide Ac-EPLPPPP-NH2 from Geobacillus thermodenitrificans

Structure of the PRO-PRO endopeptidase (PPEP-3) E153A Y189F in complex with substrate peptide Ac-EPLPPPP-NH2 from Geobacillus thermodenitrificans Descriptor: ACE-GLU-PRO-LEU-PRO-PRO-PRO-PRO-NH2, ATLF-like domain-containing protein, TETRAETHYLENE GLYCOL, ... Authors: Claushuis, B, Wojtalla, F, van Leeuwen, H, Corver, J, Baumann, U, Hensbergen, P. Deposit date: 2024-07-17 Release date: 2025-07-30 Last modified: 2026-02-18 Method: X-RAY DIFFRACTION (2 Å) Cite: Structural analyses and substrate profiling of PPEP-3 provide new insights into the molecular basis of Pro-Pro endopeptidase specificity. Iscience, 29, 2026来自嗜热脱氮芽孢杆菌（Geobacillus thermodenitrificans）的脯氨酸-脯氨酸内肽酶（PRO-PRO endopeptidase，PPEP-3）E153A Y189F突变体与底物肽Ac-EPLPPPP-NH2形成的复合物结构 结构标识：ACE-谷氨酸（GLU）-脯氨酸（PRO）-亮氨酸（LEU）-脯氨酸（PRO）-脯氨酸（PRO）-脯氨酸（PRO）-脯氨酸（PRO）-NH2 含ATLF样结构域的蛋白质、四乙二醇（TETRAETHYLENE GLYCOL）等 作者：Claushuis B、Wojtalla F、van Leeuwen H、Corver J、Baumann U、Hensbergen P 提交日期：2024-07-17 发布日期：2025-07-30 最后修改日期：2026-02-18 实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率2埃 引用文献：《PPEP-3的结构分析与底物谱表征为脯氨酸-脯氨酸内肽酶特异性的分子基础提供新见解》，《Iscience》，第29卷，2026年